AGONOTES: A Robot Annotator for Argonaute Proteins.

AGONOTES: A Robot Annotator for Argonaute Proteins.

Argonaute protein (Ago) exists in almost all organisms. In eukaryotes, functions as a system for the regulation of gene expression. In prokaryotes, it is a kind of defense against invasive alien genome. Ago system has been engineered to silence genes and genome editing and play an important role in biological studies.

With an increasing number of genomes and proteomes of various microbes become available, computational tools to identify and annotating argonaute protein is needed. We introduce AGONOTES (Argonaute Note). This is a web service designed specifically for identifying and annotating Ago. AGONOTES using BLASTP similarity search algorithm to categorize all proteins are delivered into three groups: argonaute prokaryotic protein (Pago), argonaute eukaryotic proteins (EAGO), and non-argonaute protein (non-Ago).

Argonaute proteins can then be adjusted to match the standard set Ago sequence using the program MUSCLE multiple sequence alignment. All functional domains can be further Ago curated from the alignment and visualized easily through the module Bio :: Graphic in bundles BioPerl. Compared with the existing tools such as CD-Search and the available databases such as UniProt and AGONOTES show much better performance on domain explanation, which is the basis for studying the new Ago. AGONOTES freely accessible in https://i.uestc.edu.cn/agonotes/. AGONOTES is a friendly tool for annotating Ago domain of the proteome, or a series of protein sequences.


Tuning the molecular and electronic properties of Pt (II) complex -organic have profound effects on their application in the fields of technology, medicine and engineering crystals. Here, we present a combination of infrared and Raman spectroscopic investigations on trans-PtCl2 (PEt3) 2 systematically carried out at various temperatures from 300 to 4.2 K in various spectral. Studies suggest orientational change drastically different from the group at around 180 K and 130 K in the group of ligands bound to the central Pt atom. This is accompanied by systematic strengthening of hydrogen bonds C-H ⋯ Cl in the temperature range 180-130 K.

 AGONOTES: A Robot Annotator for Argonaute Proteins.
AGONOTES: A Robot Annotator for Argonaute Proteins.

Human Peripheral Blood CD3+ T Cells

ABC-TC3994 1 vial Ask for price
Description: Human Peripheral Blood Mononuclear Cells are available as positive and negative controls for T-cell monitoring in ELISPOT, ELISA, cytokine bead array, tetramer/pentamer, and flow cytometry assays.

Dog Primary CD3+ Pan T Cells

T5495 1x10^6 cells / 1.0 ml
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CD3, Pan T cells Antibody, Concentrate

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ABC-TC4377 25M Ask for price
Description: Human CD3+ Pan T Cells are isolated from the mononuclear cell fraction by means of negative selection.andnbsp

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T-Pro EZ Protein Gel stain solution

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T-Pro LumiDura Chemiluminescence Detection Kit (ECL Kit)

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T-Pro Total Exosome Isolation reagent (from serum)

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T-Pro Total Exosome Isolation reagent (from serum)

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T-Pro Endotoxin Removal Plasmid Midi kit (25)

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T-Pro LumiLong Plus Chemiluminescence Detection Kit (ECL Kit)

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T-Pro Laemmli (SDS sample) Reagent (non-reducing 4X)

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T-Pro Gel/PCR DNA Purification Maxi Kit (10)

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T-Pro Gel/PCR DNA Purification Mini Kit (100)

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CD7 (T cells and NK cells), Antibody

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Human Primary CD3+ Blood Cells

T5401 5x10^5 cells / 1.0 ml
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Tumor-derived extracellular vesicles: insight observer effect of exosomes after irradiation.

This review article aims to overcome the kinetic of TDES on cancer cells pre and post-radiotherapy. Radiotherapy is traditionally used for the treatment of some types of cancer; However, there is growing evidence indicates that radiotherapy NTEs exerts on cells close to irradiated cells. In the tumor mass, the cells were irradiated may affect the cells in the non-irradiated in a different way.

Of note, exosomes are nano-scale particles release cells from tumor cells and play a key role in the survival, metastasis and tumor cell immunosuppression. Recent evidence suggests that irradiation has the potential to affect the dynamics of the different signaling pathways such as the exosome biogenesis. Indeed, exosomes act as a mediator between the various cell communication through the transmission of bio-molecules. Because of their important role in cancer biology, exosomes are in the limelight. TDES contains proprietary molecular signatures that they can function as a tumor biomarker in cancer diagnosis is different.

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B Cells, pan (APC)

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B Cells, pan (Biotin)

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Human CD19+ B Cells

T4123 5x10^5 cells / 1.0 ml Ask for price

B Cells Antibody (FITC)

GWB-A84CAA 100 TESTS Ask for price

CD40 B Cells Antibody

GWB-375761 0.2 mg Ask for price

Immortalized Human B Cells

CSC-I9357Z One Frozen vial Ask for price

Kappa Chain (B Cells) (AP)

MBS6122273-01mL 0.1(mL
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B Cells, pan (MaxLight 405)

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B Cells, pan (MaxLight 650)

MBS6255419-01mL 0.1(mL
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B Cells, pan (MaxLight 650)

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B Cells, pan (MaxLight 750)

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Kappa Chain (B Cells) (APC)

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Lambda Chain (B cells) (AP)

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Rat Pan B Cells antibody

10R-B124a 50 ug Ask for price
Description: Mouse monoclonal Rat Pan B Cells antibody

Rat Pan B Cells antibody

MBS531196-005mg 0.05mg
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Rat Pan B Cells antibody

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Kappa Chain (B Cells) (FITC)

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Lambda Chain (B cells) (HRP)

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CD19 Antibody (Pan B Cells)

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Lambda Chain (B cells) (FITC)

MBS6122306-01mL 0.1(mL
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Lambda Chain (B cells) (FITC)

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Kappa Chain (B Cells) (Biotin)

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Lambda Chain (B cells) (Biotin)

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Rat Splenocytes CD45+ B Cells

ABC-TC4225 1 vial Ask for price
Description: Rat spleen is mechanically dissociated into a single cell suspension. The splenocytes are enriched by density separation. CD4+ T cells, CD8+ T Cells, and CD45R+ B Cells and then isolated using specific immunomagnetic methods.

StimPack: Memory B cells, human

3660-1 100 µg + 1 µg
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3661-1 100 µg + 0.5 µg
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HARA-B Cells Complete Medium

CM-0724 125ml×4
EUR 98
Description: Cell lines complete growth medium

Mouse Splenocytes CD19+ B Cells

ABC-TC3938 1 vial Ask for price
Description: Mouse spleen is dissociated into a single cell suspension. The splenocytes are enriched by density separation. CD4+ T cells, CD8+T Cells, and CD19+ B Cells are isolated using specific immunomagnetic methods.

CD79a, B Cells Antibody, Concentrate

MAB895C 0.2ml
EUR 485

Human B Cells-Negatively Selected

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Description: Human B Cells (HBC) provide a source of human antibody producing cells. HBC contain B cells from the circulation with a variety of antibody specificities. HBC are used for antibody production, studies of antigen presentation function and many other facets of immunology. HBC are cryopreserved immediately following purification from HPBMC. Our HBC are quality tested via flow cytometry to ensure proper expression of CD19 and depletion of undesired cell types. HBC express CD19 but lack CD3, CD14 or CD56. Cell Features:HBC are cryopreserved upon isolation and purification.HBC are extensively tested for quality and optimal performance.Gentaur guarantees performance and quality.

Human Cord Blood CD19+ B Cells

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Description: Cord Blood-CD19+ B cells are positively isolated from mononuclear cells, using a direct immunomagnetic CD19 MicroBeads labeling system

Human Cord Blood CD19+ B Cells

CBCD19-C2.5M 2.5 million
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Human Cord Blood CD19+ B Cells

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Human Cord Blood CD19+ B Cells

CBCD19-F5M 5 million
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Kappa Chain (B Cells) (MaxLight 405)

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Kappa Chain (B Cells) (MaxLight 490)

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Kappa Chain (B Cells) (MaxLight 750)

MBS6236342-01mL 0.1(mL
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Kappa Chain (B Cells) (MaxLight 750)

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HEC-1-B Cells Complete Medium

CM-0100 125mL×4
EUR 98
Description: Cell lines complete growth medium

HEC-1-B Cells Complete Medium

CM-0100-125mL4 125 mL×4
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Description: Complete Growth Medium

Rat CD45Ra Antibody (B Cells Only)

GWB-6A11BA 0.25 mg Ask for price

Rat CD45Ra Antibody (B Cells Only)

GWB-D60850 0.1 mg Ask for price

Rat CD45Ra Antibody (B Cells Only)

GWB-F09251 1 mg Ask for price

HEC-1-B Cells Complete Medium

MBS2568609-4x125mL 4x125mL
EUR 155

Lambda Chain (B cells) (MaxLight 405)

MBS6193647-01mL 0.1(mL
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Lambda Chain (B cells) (MaxLight 405)

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Lambda Chain (B cells) (MaxLight 650)

MBS6225672-01mL 0.1(mL
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Lambda Chain (B cells) (MaxLight 650)

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Lambda Chain (B cells) (MaxLight 490)

MBS6204322-01mL 0.1(mL
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Lambda Chain (B cells) (MaxLight 490)

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Lambda Chain (B cells) (MaxLight 550)

MBS6214997-01mL 0.1(mL
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Lambda Chain (B cells) (MaxLight 550)

MBS6214997-5x01mL 5x0.1mL
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Lambda Chain (B cells) (MaxLight 750)

MBS6236347-01mL 0.1(mL
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Lambda Chain (B cells) (MaxLight 750)

MBS6236347-5x01mL 5x0.1mL
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Human Peripheral Blood CD19+ B Cells

ABC-TC3986 1 vial Ask for price
Description: Human Peripheral Blood Mononuclear Cells are available as positive and negative controls for T-cell monitoring in ELISPOT, ELISA, cytokine bead array, tetramer/pentamer, and flow cytometry assays.

Human B lymphocyte / GFP Stable Cells

SC060-G 2 x 106 cell/ml x 1ml
EUR 2670
Description: RFP expression stable cell line in Human B lymphocyte cells with Puromycin resistance

Human B lymphocyte / RFP Stable Cells

SC060-R 2 x 106 cell/ml x 1ml
EUR 2670
Description: GFP expression stable cell line in Human B lymphocyte cells with Puromycin resistance

B cells and T cells (Pan) mouse monoclonal antibody, clone HIS23, Supernatant

AM39050SU-N 1 ml Ask for price

CD20 (L26), B Cells Antibody, Concentrate

MAB327C 0.2ml
EUR 395

Streptococcus Group B protein (inactivated cells)

30-AS86 1 ml
EUR 155
Description: Inactivated Streptococcus Group B bacterial cell suspension

Interestingly, radiotherapy and IR can also contribute to change the dynamics of exosome secretion. Most likely, the contents of exosomes in irradiated cells was different than the exosomes derived from non-irradiated BCs. irradiated cells release exosomes with exclusive content NTEs mediate in BC. Given the variation in cell type, dose IR, and radio-resistance or radio-sensitivity of different cancers, there is, however, a contradiction in exosomes irradiation features and activities on neighboring cells.

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